Plasmid

Part:BBa_K5078010:Design

Designed by: Callie Ross   Group: iGEM24_UVU-Utah-2   (2024-10-01)


pL2 -aadA-nosZ(D.denit)-Ptc amiRNA-Psr


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 5337
    Illegal PstI site found at 6823
    Illegal PstI site found at 6916
    Illegal PstI site found at 8496
    Illegal PstI site found at 8983
    Illegal PstI site found at 9192
    Illegal PstI site found at 9362
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 6796
    Illegal NheI site found at 10008
    Illegal NheI site found at 10764
    Illegal PstI site found at 5337
    Illegal PstI site found at 6823
    Illegal PstI site found at 6916
    Illegal PstI site found at 8496
    Illegal PstI site found at 8983
    Illegal PstI site found at 9192
    Illegal PstI site found at 9362
    Illegal NotI site found at 11227
    Illegal NotI site found at 11317
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 11638
    Illegal BamHI site found at 12015
    Illegal XhoI site found at 6643
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 5337
    Illegal PstI site found at 6823
    Illegal PstI site found at 6916
    Illegal PstI site found at 8496
    Illegal PstI site found at 8983
    Illegal PstI site found at 9192
    Illegal PstI site found at 9362
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 5337
    Illegal PstI site found at 6823
    Illegal PstI site found at 6916
    Illegal PstI site found at 8496
    Illegal PstI site found at 8983
    Illegal PstI site found at 9192
    Illegal PstI site found at 9362
    Illegal NgoMIV site found at 1401
    Illegal NgoMIV site found at 1584
    Illegal NgoMIV site found at 1694
    Illegal NgoMIV site found at 9216
    Illegal NgoMIV site found at 9584
    Illegal NgoMIV site found at 9617
    Illegal NgoMIV site found at 9686
    Illegal AgeI site found at 8515
    Illegal AgeI site found at 9602
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

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Source

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References